Isolation and culture techniques of foetal calf chondrocytes
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چکیده
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Isolation and characterization of glycosaminoglycans secreted by human foetal lung type II pneumocytes in culture.
All major glycosaminoglycans except keratan sulphate are found in animal lung and pleura (Wusteman, 1972). Pleural fluids from patients with several pulmonary diseases contain mostly hyaluronic acid (Friman, Hellstrom, Juvani & Riska, 1977). The high concentration of hyaluronic acid in pleural fluid associated with mesothelioma is considered diagnostic of this disease (Havez, Degand, Boersma & ...
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چکیده ندارد.
15 صفحه اولQuantitative Analysis of the Proliferation and Differentiation of Rat Articular Chondrocytes in Alginate 3D Culture
Background: While articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. Several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. Methods: Rat articu...
متن کاملIsolation and characterization of calf liver thioredoxin.
The reduced form of thioredoxin, a low molecular weight hydrogen transport protein, was isolated from calf liver. The purification involved heat treatment and chromatography on DEAE-cellulose, Sephadex G-SO, and CM-cellulose, and it resulted in an apparently pure protein after a 4,000-fold purification with a final yield of 30%. The thioredoxin preparation obtained was homogeneous as judged by ...
متن کاملHuman foetal hepatocytes: isolation, characterization, and transplantation.
Hepatocyte transplantation has become an alternative to orthotopic liver transplantation for the treatment of liver metabolic diseases. However, there is an increasing lack of donor organs and isolated mature hepatocytes are difficult to manipulate and cannot be expanded in vitro. It is therefore necessary to find alternative sources of hepatocytes, and different approaches to evaluate the ther...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1981
ISSN: 0264-6021
DOI: 10.1042/bj1980141